论文部分内容阅读
目的研究氟化物对胸腺上皮细胞的毒性作用及对胸腺细胞发育的影响,探讨其作用机制。方法采用小鼠胸腺上皮细胞原代体外培养及与胸腺细胞混合培养技术,观察不同剂量氟化钠(NaF)对小鼠胸腺上皮细胞形态与功能的损伤情况及胸腺细胞在NaF处理的小鼠胸腺上皮细胞环境中的生长发育状况。结果染氟组可见小鼠胸腺上皮细胞胞体变圆,折光性增强,胞浆内有空泡形成,小鼠胸腺上皮细胞培养基中乳酸脱氢酶(LDH)活性增强,胸腺上皮细胞合成DNA和蛋白质的能力降低,白细胞介素1(IL-1)分泌量减少,并呈一定的剂量反应关系。不同剂量NaF处理的小鼠胸腺上皮细胞与新鲜的胸腺细胞混合培养,随着染氟剂量的增加,胸腺细胞摄取3H-Tdr和3H-Leu的能力降低,活细胞数目逐渐减少。结论高氟能够直接损伤小鼠胸腺上皮细胞、破坏胸腺微环境,进而导致胸腺细胞发育障碍,影响免疫功能。
Objective To study the toxic effects of fluoride on thymus epithelial cells and their effects on the development of thymocytes, and to explore its mechanism. Methods Primary culture of mouse thymus epithelial cells in vitro and mixed culture with thymus cells were used to observe the morphological and functional impairment of thymus epithelial cells with different doses of sodium fluoride (NaF) Epidermal growth and development in the environment. Results The results showed that the thymus epithelial cells in the fluoridated group became round, the refraction was enhanced, vacuoles were formed in the cytoplasm, the activity of lactate dehydrogenase (LDH) in the thymus epithelial cell culture medium was enhanced, and the DNA and The ability of protein decreased, interleukin 1 (IL-1) secretion decreased, and showed a dose-response relationship. Thymic epithelial cells of mice treated with different doses of NaF were mixed with fresh thymus cells. With the increase of fluoride dose, the ability of thymocytes to ingest 3H-Tdr and 3H-Leu decreased, and the number of living cells decreased gradually. Conclusion High fluoride can directly damage mouse thymus epithelial cells, destroy the thymus microenvironment, and then lead to the development of thymus disorders, affecting immune function.