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目的:研究血管内皮细胞生长因子(VEGF)-反义寡核苷酸(ASODN)转染舌癌Tca8113细胞对其VEGF表达和生长的抑制作用。方法:人工合成硫代磷酸化VEGF-ASODN,转染舌癌Tca8113细胞,24h后,应用原位杂交及免疫细胞化学法检测细胞VEGF mRNA及蛋白表达,ELISA法检测培养液上清中VEGF蛋白含量,流式细胞仪检测细胞凋亡情况,用MTT实验检测转染对细胞活性的影响,用细胞生长曲线表示转染对细胞生长的影响。结果与对照组比较,采用SPSS12.0软件包进行统计学分析。结果:VEGF-ASODN能显著降低VEGF mRNA及蛋白水平,降低培养液中VEGF蛋白含量,增加细胞凋亡,抑制细胞活性及生长(P<0.05)。结论:VEGF-ASODN转染舌鳞癌Tca8113细胞,能显著抑制VEGF mRNA及蛋白表达,增加细胞凋亡,抑制细胞生长。
AIM: To investigate the inhibitory effect of vascular endothelial growth factor (VEGF) - antisense oligonucleotide (ASODN) transfected Tca8113 cells on the VEGF expression and growth. Methods: VEGF-ASODN was synthesized and transfected into tongue cancer Tca8113 cells. After 24h, the expression of VEGF mRNA and protein was detected by in situ hybridization and immunocytochemistry. VEGF protein level The apoptosis of cells was detected by flow cytometry. The effect of transfection on cell viability was detected by MTT assay. The cell growth curve was used to show the effect of transfection on cell growth. Results Compared with the control group, SPSS12.0 software package was used for statistical analysis. Results: VEGF-ASODN could significantly decrease the level of VEGF mRNA and protein, decrease the content of VEGF protein in the culture medium, increase the apoptosis and inhibit the cell activity and growth (P <0.05). CONCLUSION: VEGF-ASODN transfected Tca8113 cells can significantly inhibit the expression of VEGF mRNA and protein, increase apoptosis and inhibit cell growth.