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参试的盆栽柑桔树为嫁接在一年半生Rusk citrange砧木上的一年生“Valencia”橙,在控制环境中连续保持六星期,以诱导耐寒力;耐寒锻炼的温度为10±1℃、照度为500微爱因斯坦/米~2/秒（光合活动辐射,简称PAR）、相对湿度为60±5％。在上述条件下经过五星期的逐步耐寒锻炼以后,结果能忍耐-6.7℃低温达4小时而无明显寒害。叶片需经五星期耐寒锻炼后才不受害,茎部需经三星期耐寒锻炼后才不受害。第一星期由于碳水化合物积累结果,叶片中容质增加最快。 脯氨酸、谷氨酸和缬氨酸增加,其它氨基酸反而减少。经六星期耐寒锻炼后,锻炼植株的叶片持水量平均为-19.5巴,而未经锻炼的对照树叶片的持水量为-5巴。
The potted citrus tree was an annual “Valencia” orange grafted on Rusk citrange rootstock for one year and a half and kept in control for six weeks in a row to induce cold resistance; the temperature of the hardiness exercising was 10 ± 1 ° C and the illuminance was 500 micro-Einstein / m ~ 2 / s (photosynthetic active radiation, referred to PAR), relative humidity of 60 ± 5%. After five weeks of gradual cold-resistance exercise under the above conditions, the results were able to endure the hypothermia at -6.7 ° C for 4 hours without significant cold damage. After five weeks of cold hardiness, the leaves need not be harmed. The stems need not suffer from cold for three weeks. The first week due to the accumulation of carbohydrates, leaf content in the fastest increase. Proline, glutamate and valine increased, while other amino acids decreased. After six weeks cold-resistant exercise, the leaf water holding capacity of the plants was -19.5 bar on average, while that of the untreated control leaves was -5 bar.