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本文应用ELISA方法检测38例重症肌无力(MG)患者血清中抗乙酰胆碱受体抗体(AchRab),并设正常人、其他疾病(神经系统)对照组,现将观察结果报道如下。资料和方法原理:ELISA方法检测抗乙酰胆碱受体抗体的实验原理是用酶促反应的放大作用来显示初级免疫反应。一、试剂 1.包被液:0.05MpH9.6碳酸盐缓冲液。 2.洗涤
In this paper, 38 cases of myasthenia gravis (MG) serum anti-acetylcholine receptor antibody (AchRab) was detected by ELISA, and normal subjects, other diseases (nervous system) control group, the observations are reported below. Materials and Methods Principle: ELISA method to detect anti-acetylcholine receptor antibody experimental principle is the use of enzymatic amplification to show the primary immune response. A reagent 1 coating solution: 0.05MpH9.6 carbonate buffer. Wash