论文部分内容阅读
以前的工作表明，高钾暴露可以导致蛙骨骼肌细胞二酰基甘油（ＤＡＧ）累积和蛋白激酶Ｃ（ＰＫＣ）激活。为探讨其作用机制，进一步研究了咖啡因对ＤＡＧ／ＰＫＣ信号转导途径的影响。结果表明，咖啡因能以浓度和时间依赖的方式引起蛙骨骼肌ＤＡＧ累积和ＰＫＣ激活。在肌浆自由钙升高达到相同峰值条件下，咖啡因引起的ＤＡＧ累积程度和时相明显不同于高钾。与此同时，咖啡因引起的ＰＫＣ激活也不同于高钾。咖啡因与高钾作用的差别进一步证实，对于蛙骨骼肌ＤＡＧ／ＰＫＣ信号转导系统的调控，除了肌浆自由钙升高外，高钾暴露引起的膜去极化可能也起作用。
Previous work has shown that high potassium exposure can lead to the accumulation of diacylglycerol (DAG) and activation of protein kinase C (PKC) in frog skeletal muscle cells. In order to explore its mechanism of action, we further studied the effect of caffeine on DAG / PKC signal transduction pathway. The results show that caffeine induces DAG accumulation and PKC activation in frog skeletal muscle in a concentration and time-dependent manner. In the sarcoplasmic free calcium up to the same peak conditions, caffeine-induced accumulation of DAG and the phase is significantly different from the high potassium. At the same time, caffeine-induced PKC activation is also different from high potassium. The difference between caffeine and high potassium further confirms that in addition to the increase of sarcoplasmic free calcium, membrane depolarization caused by high potassium exposure may also play a role in the regulation of the DAG / PKC signal transduction system in frog skeletal muscle.