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目的:研究孕中期乙型肝炎孕妇血清白细胞介素-21(IL-21)、甲胎蛋白(AFP)水平变化及相关关系。方法:采用实时荧光PCR检测69例乙型肝炎孕妇HBV DNA并分组,HBV DNA<103copies/ml为A组,103copies/ml≤HBV DNA<106copies/ml为B组,HBV DNA≥106copies/ml为C组。采用酶联免疫吸附法(ELISA)检测血清IL-21,电化学发光法检测血清AFP,并与30例正常孕妇对照组比较。结果:B、C组血清IL-21水平高于A组和对照组,差异有统计学意义(P<0.05),C组与B组差异无统计学意义(P>0.05);B组血清AFP水平高于对照组和A组,差异有统计学意义(P<0.05);C组血清AFP水平高于其他3组,差异有统计学意义(P<0.05)。乙型肝炎孕妇组血清IL-21与AFP呈正相关(P<0.05)。结论:乙型肝炎孕妇血清IL-21与肝脏炎症密切相关,可能参与机体免疫应答,且与AFP有一定相关性,联合检测血清IL-21和AFP的表达可减少乙型肝炎对孕妇产前筛查结果的影响。
Objective: To study the changes of serum interleukin-21 (IL-21) and alpha-fetoprotein (AFP) in pregnant women with second trimester of pregnancy. Methods: The HBV DNA of 103 pregnant women with HBV DNA less than 103copies / ml were divided into group A and group B with 103copies / ml≤HBV DNA <106copies / ml, HBV DNA≥106copies / ml was C group. Serum IL-21 was detected by enzyme-linked immunosorbent assay (ELISA), serum AFP was detected by electrochemiluminescence, and compared with 30 normal pregnant women control group. Results: The levels of serum IL-21 in group B and group C were significantly higher than those in group A and control (P <0.05), but there was no significant difference between group C and group B (P> 0.05) (P <0.05). The level of serum AFP in group C was higher than that in the other three groups (P <0.05). Serum levels of IL-21 in hepatitis B pregnant women were positively correlated with AFP (P <0.05). Conclusion: The serum level of IL-21 in hepatitis B pregnant women is closely related to liver inflammation, which may be involved in the immune response and has a certain correlation with AFP. Combined detection of serum IL-21 and AFP may reduce the prenatal screening of hepatitis B Check the results of the impact.