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本文采用苯巴比妥掺食法，连续给药８０ｄ，造成大鼠身体依赖性模型。自然戒断后第１４ｈ，开始出现戒断症状，并逐渐加重，第１６ｈ出现震颤、抽搐等症状，第２４ｈ出现阵发自发性惊厥，部分大鼠因惊厥而死亡。第６２ｈ症状完全自然消退。与苯巴比妥等药效剂量的安定可以部分抑制戒断症状。用荧光法对大鼠脑内的单胺类递质的测定表明：５—ＨＴ、５—ＨＩＡＡ，ＮＡ和ＤＡ的含量在依赖状态或是戒断后第７２ｈ与对照组比较，无明显的差异。用放射受体法测定苯巴比妥依赖及戒断状态大鼠脑内ＧＡＢＡ的含量，结果表明：二者ＧＡＢＡ值明显地低于空白对照组，安定替代组大鼠脑内ＧＡＢＡ含量亦显著地低于对照组。
In this paper, phenobarbital mixed with law, continuous administration 80d, resulting in rat body-dependent model. At 14h after natural withdrawal, withdrawal symptoms began to appear, and gradually increased, the first 16h tremor, convulsions and other symptoms, intermittent spontaneous seizures occurred at 24h, some rats died of convulsions. The symptoms disappeared completely at 62h. Diazepam and other pharmacodynamic doses of diazepam can partially inhibit withdrawal symptoms. Fluorescence assay of monoamine neurotransmitters in rat brain showed that the content of 5-HT, 5-HIAA, NA and DA in the dependent state or 72h after withdrawal compared with the control group, no significant difference . The contents of GABA in the brain of phenobarbital-dependent and withdrawal rats were determined by radioimmunoassay. The results showed that the GABA values of the two groups were significantly lower than those of the blank control group, and the content of GABA in the brain of the stable-substitution group was also significantly Lower than the control group.