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甲苯作为土壤酶测定的前处理试剂及许多有机污染物在环境中降解的中间产物,有关其对土壤酶的影响研究在土壤酶学和环境科学领域具有重要意义.因此,采用模拟方法,较系统地研究了不同剂量甲苯和处理时间下芳基硫酸酯酶活性的变化规律.结果表明,甲苯对纯酶具有明显的抑制作用,酶活性降幅最大达到45.5%;灭菌土壤对溶液中的纯酶有很强的吸附能力;极微量的甲苯即可完成对土壤中酶活性的激活作用,酶活性增幅为109%～298%;随着甲苯剂量的增加,土壤酶活性的变化幅度逐渐趋缓,并可用Langmuir模型较好地拟合,由此获得了最大表观酶活性Umax,发现其与土壤性质显著相关,说明甲苯主要是通过杀死土壤中的微生物来影响土壤酶活性的.此外,初步探讨了不同土壤中胞外酶量与胞内酶量的关系,发现在供试土样中土壤芳香硫酸酯酶胞外酶和胞内酶分别占54.4%和45.6%.本研究可为后续土壤酶测定质量的完善和提高提供依据.
Toluene as a pretreatment reagent for determination of soil enzymes and intermediates for the degradation of many organic pollutants in the environment are of great significance in the field of soil enzymology and environmental science.Therefore, The changes of aryl sulfatase activity under different doses of toluene and treatment time were studied.The results showed that toluene had obvious inhibition on pure enzyme and the maximum decrease reached 45.5% A very small amount of toluene to complete the activation of soil enzyme activity, an increase of 109% to 298% of enzyme activity; with the increase of toluene dose, the change of soil enzyme activity gradually slowed down, And can be better fitted by Langmuir model, thus obtaining the maximum enzyme activity Umax, was found to be significantly correlated with soil properties, indicating that toluene mainly through the killing of soil microorganisms to affect soil enzyme activity.In addition, the initial The relationship between the amount of extracellular enzyme and the amount of intracellular enzyme in different soils was investigated and it was found that the extracellular and intracellular enzymes of soil aroma sulfatase accounted for 54.4% and 45.6% respectively The research can provide the basis for the improvement and improvement of the quality of subsequent soil enzyme determination.