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糖尿病心肌病是极度危险的糖尿病并发症之一,它的发病机制目前尚未明确。本研究采用30只Sprague-Dawley大鼠随机分为2组:糖尿病大鼠组(15只),尾静脉注射链脲佐菌素造模,正常组大鼠(15只),尾静脉注射生理盐水,成模4周后采用苏木精-伊红染色及透射电子显微镜观察两组大鼠心肌细胞纤维显微结构和超微结构的病理改变,同时免疫组织化学SABC法对糖尿病大鼠与正常大鼠心肌细胞中凝血酶1(TSP-1)和转化生长因子β(TGF-β)的表达进行观察。糖尿病大鼠成模前,两组动物的体重、血糖和尿糖检测结果间无显著差异(P>0.05)。成模四周后,糖尿病大鼠与正常大鼠体重、血糖和尿糖检测结果有显著性差异(P<0.01)。光镜和电子显微镜观察显示糖尿病大鼠心肌组织结构有明显改变。糖尿病大鼠心肌细胞内TSP-1和TGF-β的表达显著增强(P<0.01)。本研究结果表明TSP-1和TGF-β在糖尿病大鼠的表达增加可能是糖尿病心肌病发病的一个重要因素。
Diabetic cardiomyopathy is one of the most dangerous complications of diabetes. Its pathogenesis is not yet clear. In this study, 30 Sprague-Dawley rats were randomly divided into two groups: diabetic rats (15), tail vein injection of streptozotocin model, the normal group of rats (15), tail vein injection of saline After 4 weeks of operation, hematoxylin-eosin staining and transmission electron microscopy were used to observe the pathological changes of myocardial ultrastructure and ultrastructure of myocardial cells of rats in both groups. Immunohistochemical SABC method was used to detect the changes of myocardial ultrastructure in normal rats The expression of thrombin-1 (TSP-1) and transforming growth factor-β (TGF-β) in rat cardiomyocytes was observed. There was no significant difference in body weight, blood glucose and urine glucose between the two groups before modeling (P> 0.05). Four weeks after the model was established, there was a significant difference in body weight, blood glucose and urine glucose between diabetic rats and normal rats (P <0.01). Light microscopy and electron microscopy showed that the structure of myocardium in diabetic rats changed significantly. The expression of TSP-1 and TGF-β in diabetic rat myocardial cells was significantly increased (P <0.01). The results of this study indicate that the increased expression of TSP-1 and TGF-β in diabetic rats may be an important factor in the pathogenesis of diabetic cardiomyopathy.