为深入理解肿腿蜂雌性抚育的行为学特征,在室内连续观察了雌性管氏肿腿蜂Sclerodermus guani对子代幼虫的转移行为,旨在明确雌蜂在子代蜂幼虫发育到哪一阶段时启动转移行为,以及幼虫转移行为是否有节律。以黄粉虫Tenebrio militor蛹期在24h内的蛹体为寄主,根据子代蜂幼虫发育进程将其划分为低龄幼虫(1-2龄)、高龄幼虫(3-4龄)、老熟幼虫(自然脱落)和吐丝幼虫(开始吐丝结茧)等4个时期,采取人工剥离(早期幼虫)或自然脱离(晚期幼虫)的方法处理子代蜂幼虫,观察雌蜂对所表现出的行为反应;然后以子代蜂高龄幼虫为对象,连续观察雌蜂的30次幼虫转移行为过程。结果表明:雌蜂对所有发育时期子代蜂幼虫均用触角拍打进行探测;但不转移低龄幼虫,只转移其他阶段幼虫,转移老熟幼虫和吐丝幼虫的瞬间概率分别是转移高龄幼虫的4.09倍和7.69倍。雌蜂转移高龄、老熟和吐丝幼虫的比例分别为96%,100%和100%,没有显著差异(P≥0.05);对高龄幼虫、老熟幼虫和吐丝幼虫转移耗时平均分别为27.96,34.04和32.49s,没有显著差异(P≥0.05);平均转移距离依次为4.19,7.18和9.43mm,对吐丝幼虫的转移距离显著大于高龄幼虫(P<0.05),但在高龄和老熟幼虫之间没有显著差异(P≥0.05)。对雌蜂连续30次幼虫转移行为的趋势和节律分析表明:幼虫转移前探测的幼虫数总体上随幼虫转移次序增加而减少,在间隔1次和2次之间存在显著自相关,幼虫转移耗时在间隔1次之间存在显著自相关,但幼虫转移距离未表现出明显的节律。本研究结果说明,管氏肿腿蜂雌性抚育中的幼虫转移行为只在子代蜂幼虫发育到较高龄期时启动,且幼虫转移中的某些行为特征具有节律性。 In order to understand the behavioral characteristics of female nurture, the effects of Sclerodermus guani on the offspring larvae were observed continuously in the laboratory. Start transfer behavior, and larval transfer behavior is rhythmic. The pupae of the Tenebrio militor pupal stage within 24 hours were used as hosts. The larvae were divided into two groups: young larvae (1-2 years old), young larvae (3-4 years old), mature larvae (Larvae) or spontaneous detachment (late larvae) were used to deal with the parasitoid larvae and observe the behavioral responses of female bees Then, the larvae of the adult male bee were used as the targets to observe the transfer behavior of the female larvae for 30 larvae. The results showed that the female bees detected the bee larvae at all developmental stages by beeswaxing. However, the instantaneous probability of transferring the larvae of other stages to the mature larvae and larvae without transferring the young larvae was 4.09 Times and 7.69 times. There was no significant difference (P≥0.05) in the percentage of female bee transferring old, mature and filarial larvae, respectively. The average time for the transfer of larvae, mature larvae and larvae was 27.96, 34.04 and 32.49 s, respectively. There was no significant difference between them (P> 0.05). The average transfer distance was 4.19, 7.18 and 9.43 mm respectively. The transfer distance to Larval was significantly longer than that of the older larva (P <0.05) There was no significant difference between mature larvae (P> 0.05). The tendency and rhythm analysis of the female bee larvae for 30 successive larvae transfer showed that the number of larvae detected before larvae transfer generally decreased with the increase of larvae transfer order. There was a significant autocorrelation between interval 1 and 2 larvae There was significant autocorrelation between intervals, but the larval migration distance did not show obvious rhythm. The results of this study suggest that the larval transfer behavior of female Larvae in Schistosoma japonicum only starts when the parasitoid larvae develop to a higher age, and some of the behavioral characteristics of larval metastasis are rhythmic.