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目的:探讨在体外肝脏灌注(extracorporealliverperfusion,ECLP)中影响供肝功能的因素。方法:实验动物均为健康普通长白猪,随机分为4组。A组(n=4)供肝灌注时保存在35℃～36℃的保存液中;B组(n=4)在ECLP循环中不加入胰岛素和50%高糖;C组(n=4)供肝在进行ECLP循环时仅灌注门静脉;D组(n=7)同时灌注门静脉和肝动脉,ECLP循环中每小时加入胰岛素和50%葡萄糖,而且供肝灌注时保存在38℃～39℃的保存液中。A、B和C组在分组条件以外其他条件均与D组相同。观察供肝一般情况、胆汁生成量、耗氧率、病理变化等指标。结果:各组胆汁生成量和耗氧率均逐渐下降而灌注压力逐渐升高。A组、D组耗氧率和C组、D组胆汁生成量及耗氧率的差异均有统计学意义(P<0.05),而B组、D组间各指标差异无统计学意义。结论:体外肝脏功能的影响因素是多方面的,其中保存温度和灌注方式可能是较重要的因素。
Objective: To investigate the factors that affect hepatic function in extracorporeal bowelperfusion (ECLP). Methods: All the experimental animals were healthy common Landrace and were randomly divided into 4 groups. In group A (n = 4), the donor liver was preserved in preservation solution at 35 ° C to 36 ° C. In group B (n = 4), no insulin and 50% The portal vein was infused only in the ECLP circulation. In the D group (n = 7), the portal vein and the hepatic artery were infused at the same time. Insulin and 50% glucose were added to the ECLP circulation every hour. The donor liver was preserved at 38 ° C to 39 ° C Save the solution. Groups A, B, and C have the same conditions other than grouping as group D. Observed the general situation of liver, bile production, oxygen consumption, pathological changes and other indicators. Results: The bile production and oxygen consumption rate of each group decreased gradually and the perfusion pressure gradually increased. The oxygen consumption rate of group A and group D was significantly different from those of group C and group D (P <0.05), but there was no significant difference between group B and group D (P> 0.05). Conclusion: There are many influencing factors of liver function in vitro, of which storage temperature and perfusion may be the more important factors.