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目的检测地榆皂苷II对多种肿瘤细胞的体外增殖抑制作用,研究地榆皂苷II诱导肿瘤细胞凋亡的分子机制。方法采用SRB法检测地榆皂苷II对肿瘤细胞的体外增殖抑制,通过Western blot来研究地榆皂苷II对三大凋亡通路的影响。结果地榆皂苷Ⅱ可上调MDA-MB-231细胞中活性氧的水平,上调CHOP的表达,激活内质网应激途径;下调Bcl-2的表达,上调Bax的表达,激活线粒体凋亡途径;上调DR4、DR5的表达,激活TRAIL外源凋亡通路。结论地榆皂苷II通过激活三大细胞凋亡通路来抑制肿瘤细胞的增殖,促进肿瘤细胞的凋亡。
Objective To detect the inhibitory effect of saponin II on the proliferation of various tumor cells in vitro and to study the molecular mechanism of the apoptosis induced by Saponin II in tumor cells. Methods The inhibitory effect of saponin II on proliferation of tumor cells was detected by SRB method. The effects of saponin II on apoptosis pathway were studied by Western blot. Results Sarbolins Ⅱ could up-regulate the level of reactive oxygen species (ROS) in MDA-MB-231 cells, up-regulate the expression of CHOP and activate the endoplasmic reticulum stress pathway, down-regulate the expression of Bcl-2 and up- Upregulate the expression of DR4 and DR5 and activate the TRAIL extrinsic apoptotic pathway. Conclusion SULP II can inhibit the proliferation of tumor cells and promote the apoptosis of tumor cells by activating the apoptosis pathway of the three major cells.