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目的观察一种新的活化T细胞膜分子p140的分布、相对分子质量(Mr)和功能。方法用免疫荧光染色和流式细胞术观察p140分子的分布;采用重导向杀伤实验观察其对杀伤功能的调节;应用生物素标记细胞膜分子、免疫沉淀及化学发光测定p140的Mr。结果p140分子可选择性地表达于混合淋巴细胞培养(MLC)所产生的活化T细胞表面,而在抗CD3mAb、PHA、PMA或PMA+A23187几种刺激剂活化的人外周血单个核细胞(PBMC)上未见表达。p140还分布于一些人急性T细胞性白血病细胞系。在重导向杀伤实验中,p140与CD3分子有协同作用。p140Mr为140000。结论p140可能为一种新的移植抗原诱导的T细胞活化分子,同CD3分子具有协同刺激作用。
Objective To observe the distribution, relative molecular mass (Mr) and function of a novel activated T cell membrane molecule p140. Methods The distribution of p140 was observed by immunofluorescence staining and flow cytometry. The killing effect of p140 was observed by using the redirecting and killing experiments. The biotin-labeled cell membrane molecules, immunoprecipitation and chemiluminescence were used to determine the expression of p140. Results The p140 molecule was selectively expressed on the surface of activated T cells produced by mixed lymphocyte culture (MLC), whereas the activation of p140 on PBMCs stimulated with anti-CD3 mAb, PHA, PMA or PMA + A23187 stimuli ) No expression. p140 is also distributed in some human acute T cell leukemia cell lines. In redirecting experiments, p140 and CD3 molecules have a synergistic effect. p140Mr is 140000. Conclusion p140 may be a new T cell activation molecule induced by transplantation antigen and has a synergistic stimulating effect with CD3 molecule.