目的建立测定人血浆中尼美舒利浓度的方法。方法采用反相高效液相色谱法。结果尼美舒利在0.4~12.8μg/ml范围内有良好线性关系,r=0.9997,血浆中药物最低检测浓度为0.15μg/ml,血浆中3种浓度0.4、3.2、12.8μg/ml回收率分别为99.8%、101.0%、100.5%,日内、日间RSD分别为5.58%、2.97%、2.22%及5.54%、3.82%、2.91%。结论本法操作简便、结果可靠,适用于尼美舒利血药浓度及生物利用度的测定。 Objective To establish a method for the determination of nimesulide concentration in human plasma. Methods Using reversed-phase high performance liquid chromatography. Results Nimesulide had a good linearity in the range of 0.4 ~ 12.8μg / ml, r = 0.9997, the lowest concentration of drug in plasma was 0.15μg / ml, plasma concentrations of 0.4, 3.2, 12.8μg / ml Respectively, 99.8%, 101.0% and 100.5% respectively. The intra-day and interday RSDs were 5.58%, 2.97%, 2.22% and 5.54%, 3.82% and 2.91%, respectively. Conclusion The method is simple, reliable and suitable for the determination of nimesulide plasma concentration and bioavailability.