论文部分内容阅读
目的对紫外速率定量法和改良G6PD/6PGD比值法检测女性杂合子G6PD活性进行对比分析和评价,确定出对G6PD缺乏的女性杂合子检出率较高的筛查方法。方法对突变特异性扩增系统(ARMS)法确诊的60例G6PD缺乏女性杂合子及60例G6PD正常病例,分别采用紫外速率定量法和改良G6PD/6PGD比值法进行检测,并对结果进行对比分析。结果紫外速率定量法的灵敏度为65.0%,特异度为96.7%,改良G6PD/6PGD比值法的灵敏度为38.3%,特异度为91.7%,两者对G6PD缺乏的女性杂合子的检出率差异有统计学意义(P<0.01)。结论紫外速率定量法是筛检G6PD缺乏症较理想的方法,但仍然存在一定的漏检率。
Objective To compare and evaluate the G6PD activity of heterozygous female mice by UV quantification and modified G6PD / 6PGD ratio method, and to identify the screening method for the detection rate of heterozygous female heterozygotes in G6PD deficiency. Methods Sixty cases of G6PD-deficient female heterozygotes and 60 cases of G6PD normal cases diagnosed by mutation-specific amplification system (ARMS) were detected by UV quantitative method and modified G6PD / 6PGD ratio method respectively, and the results were compared . Results The sensitivity and specificity of the UV quantitative method were 65.0% and 96.7%, respectively. The sensitivity and specificity of the modified G6PD / 6PGD method were 38.3% and 91.7% respectively. There was a significant difference between the two methods in the detection rate of G6PD deficiency in female heterozygotes Statistical significance (P <0.01). Conclusion UV quantification is an ideal method to screen G6PD deficiency, but there is still a certain missing rate.