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将带有二叶一心的小苗扦插于附加不同浓度 Na Cl的 MS培养基中 ,研究不同番茄材料发根、植株生长以及 POD酶酶谱及其活性的变化 ,并通过花粉管导入技术将野生番茄的总 DNA导入到栽培种。研究结果表明 ,Lycopersicon pennellii LA71 6和 Lycopersicon pimpinellifoliumLA2 1 84始终具有相对高的生根率 ,在 1 50 mmol/L Na Cl胁迫 1 0 d时 ,相对发根率分别为 86.8%和1 0 0 %。参试的 4份野生种 ,在附加有 75mmol/L Na Cl的培养基上的株高和叶片数均高于对照 (无Na Cl) ,随着 Na Cl浓度的不断提高 ,株高和叶片数都下降 ,Lycopersicon pennellii LA71 6下降幅度最小。 4份野生种的耐盐性明显优于 2份栽培种 ,当 Na Cl浓度提高到 30 0 mmol/L时 ,4份野生种的鲜重可达对照的 0 .90、1 .0 7、1 .60和 1 .53倍 ,而 2份栽培种则为对照的 0 .55和 0 .33倍。当Na Cl浓度为 75mmol/L时 ,Lycopersicon peruvianum LA1 1 1和 Lycopersicon pennellii LA71 6材料 POD酶酶带数多于对照 2条和 1条 ,Lycopersicon cheesmanii LA1 66和 Lycopersicon pimpinellifoliumLA2 1 84则少于对照 1条和 3条。利用花粉管导入技术可以将野生番茄的 DNA导入到栽培番茄。
The seedlings with two leaves were cut into MS medium supplemented with NaCl at different concentrations to study the changes of hairy root, plant growth and POD zymogram and activity of different tomato materials. The pollen tube introduction technology was used to transform wild tomato Of the total DNA into cultivars. The results showed that Lycopersicon pennellii LA71 6 and Lycopersicon pimpinellifolium LA2 1 84 always had a relatively high rooting rate, and the relative rooting rates were 86.8% and 100% respectively under the stress of 10 mmol / L NaCl for 10 days. The plant height and leaf number of the 4 wild species tested in the medium supplemented with 75 mmol / L NaCl were higher than those of the control (no NaCl). With the continuous increase of NaCl concentration, the plant height and number of leaves Fell, Lycopersicon pennellii LA71 6 decreased the least. The salt tolerance of four wild species was significantly better than that of two cultivars. When NaCl concentration was increased to 300 mmol / L, the fresh weight of four wild species reached 0 .90, 1 .0 7, 1 .60 and 1.53 times, while the two cultivars were 0.55 and 0.33 times of the control. When the concentration of NaCl was 75mmol / L, the number of POD enzyme bands in Lycopersicon peruvianum LA1 1 1 and Lycopersicon pennellii LA71 6 materials were more than those in control 2 and 1, but Lycopersicon cheesmanii LA1 66 and Lycopersicon pimpinellifolium LA2 1 84 were less than that in control 1 And three. Wild tomato DNA can be introduced into cultivated tomatoes using the pollen tube introduction technique.