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目的为提高发热伴血小板减少综合征早期诊断的准确性。方法收集RT-PCR阴性的发热伴血小板减少综合征确诊病例首份血清以及恢复期血清各10份,采用套式RT-PCR检测特异性核酸;ELISA方法检测急性期与恢复期血清中特异性抗体Ig G。结果 10份血清均能扩增到新型布尼亚病毒的S、M、L 3个特异性基因片段。10份恢复期血清中均能检测到高浓度的特异性抗体Ig G,其S/CO值分布在23.88~30.69。结论 RT-PCR在诊断发热伴血小板减少综合征疑似病例的急性期血清中存在着误诊现象,应引起临床与流行病学医生重视。套式RT-PCR方法灵敏、特异性高,可作为此类标本的补充试验以降低发热伴血小板减少综合征的误诊率。
The purpose is to improve the accuracy of early diagnosis of fever with thrombocytopenia syndrome. Methods RT-PCR negative samples of fever and thrombocytopenia syndrome were collected for the first sera and convalescent sera, respectively. The specific nucleic acids were detected by nested RT-PCR. The serum levels of specific antibodies Ig G. Results All the 10 serum samples were able to amplify S, M, L specific gene fragments of new Bunyavirus. 10 convalescent serums could detect high concentrations of specific antibodies Ig G, the S / CO value distribution in 23.88 ~ 30.69. Conclusion RT-PCR is a misdiagnosis in the diagnosis of fever in patients with suspected cases of thrombocytopenia syndrome, which should be paid more attention by clinical and epidemiological doctors. The nested RT-PCR method is sensitive and specific and can be used as a supplementary test to reduce the misdiagnosis rate of fever with thrombocytopenia syndrome.