论文部分内容阅读
目的：探讨免疫组化检测ｐ５３蛋白表达和ＰＣＲ－ＳＳＣＰ检测ｐ５３基因突变的一致性。方法：单克隆抗体ＤＯ－７检测８５例非小细胞肺癌（ＮＳＣＬＣ）的ｐ５３蛋白表达，ＰＣＲ－ＳＳＣＰ检测其中３１例腺癌的ｐ５３基因突变。结果：８５例ＮＳＣＬＣ中ｐ５３蛋白表达阳性率为６８％（５８／８５），３１例腺癌中ｐ５３蛋白表达阳性率为６１％（１９／３１），１４例（４６％）出现ｐ５３基因５～８外显子突变，ｐ５３蛋白免疫组化和ＰＣＲ－ＳＳＣＰ检测ｐ５３基因突变无显著相关（χ２＝０．１，Ｐ＝０．７６），其一致率为５２％。结论：ｐ５３蛋白表达并不能很好地反映ｐ５３基因突变。
OBJECTIVE: To investigate the consistency of p53 protein expression detected by immunohistochemistry and p53-p53 mutation detected by PCR-SSCP. Methods: p53 protein was detected by monoclonal antibody DO-7 in 85 cases of non-small cell lung cancer (NSCLC). P53 gene mutation was detected in 31 cases of adenocarcinoma by PCR-SSCP. Results: The positive rate of p53 protein expression was 68% (58/85) in 85 cases of NSCLC, and 61% (19/31) in 31 cases of adenocarcinoma. The p53 gene was found in 14 cases (46% 8 exon mutation, p53 protein immunohistochemistry and PCR-SSCP detection of p53 gene mutation was no significant correlation (χ2 = 0.1, P = 0.76), the concordance rate was 52%. Conclusion: p53 protein expression does not reflect the p53 gene mutation well.