为建立快速、有效的青花菜根肿病苗期抗性鉴定技术,将芸薹根肿菌Plamodiophora brassicae Woron.人工接种于高感根肿病青花菜自交系90196,研究了接种菌液浓度、接种寄主苗龄、接种基质p H和接种方法等对人工接种鉴定效果的影响。结果表明,在一定范围内,根肿病发病率及病情指数随着接种菌液浓度的升高而增大,接种菌液浓度为3×108CFU/m L时,发病率及病情指数分别为97.22%和86.11,可以反映寄主真实的抗性水平;接种寄主苗龄为2~6叶期均能使植株发病,但2~3叶期发病效果最佳;接种基质偏酸性(p H 5~6)有利于根肿病的发生;使用伤根灌菌法进行鉴定,青花菜根肿病发病率和病情指数均最高,分别为100.00%和88.10,优于蘸根法和浸芽法。用已知抗性水平的12个自交系和8个杂交种进行验证,鉴定结果表明该苗期抗性鉴定技术可客观反映供试材料的实际抗性水平。 In order to establish a rapid and effective technique for identifying resistance of broccoli clubhead at seedling stage, Plamodiophora brassicae Woron. Was inoculated on inbred line Brineut Highland 90196 with high susceptibility to clubroot, and the inoculation bacterial concentration and inoculation Host seedling age, inoculant matrix p H and inoculation methods on the effect of artificial inoculation. The results showed that in a certain range, the incidence and the disease index of clubroot disease increased with the increasing of inoculum concentration. The inoculum concentration of 3 × 108CFU / m L, the incidence and disease index were 97.22 % And 86.11, respectively, could reflect the true resistance level of the host. The seedling age of 2 to 6 days after inoculation of the host could make the plant disease, but the best effect occurred in the second to third leaf stage. ) Was favorable to the occurrence of clubroot disease. The identification of root ganglion method showed that the incidence and index of broccoli clubroot were the highest (100.00% and 88.10 respectively), which was better than dipping and bud bud method. 12 inbred lines and 8 hybrids with known resistance levels were used for validation. The identification results showed that the seedling resistance evaluation technique can objectively reflect the actual resistance level of the tested materials.