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目的 探讨神经生长因子 (NGF)在哮喘发病中的作用及其神经免疫调节机制。 方法 应用原位杂交方法结合显微图像分析 ,研究实验性哮喘豚鼠下呼吸道和内脏感觉传入部位肿瘤坏死因子 αmRNA(TNF αmRNA)表达的变化以及NGF抗体对其的影响。 结果 生理盐水组与单纯致敏组TNF αmRNA的表达没有显著差异 (P >0 0 5 ) ;与这两个对照组相比 ,哮喘豚鼠TNF αmRNA的表达在气道上皮、肺内炎性细胞、C7～T5段脊神经节及脊髓后角内均明显上调 (P <0 0 1) ;在哮喘 +NGF抗体组 ,上述部位内TNF αmRNA的表达明显低于哮喘组 (P <0 0 1)。 结论 NGF诱发TNF α的表达可能是NGF参与哮喘发病的机制之一 ,NGF抗体抑制哮喘时下呼吸道和内脏感觉传入部位TNF αmRNA的表达可能是治疗哮喘的新途径。
Objective To investigate the role of nerve growth factor (NGF) in the pathogenesis of asthma and its neural-immune regulatory mechanism. Methods In situ hybridization and microscopic image analysis were used to investigate the changes of tumor necrosis factor α mRNA (mRNA) expression in the lower respiratory tract and visceral afferent sites of experimental asthmatic guinea pigs and the effect of NGF antibody. Results There was no significant difference in the expression of TNFαmRNA in the normal saline group and the sensitized group (P> 0.05). Compared with the two control groups, the expression of TNFαmRNA in the asthmatic guinea pigs was significantly increased in the airway epithelium, pulmonary inflammatory cells, In C7 ~ T5 spinal ganglia and spinal dorsal horn were significantly increased (P <0.01); in the asthma + NGF antibody group, the expression of TNFαmRNA in the above sites was significantly lower than that in the asthma group (P <0.01). Conclusion NGF-induced TNFα expression may be one of the mechanisms involved in the pathogenesis of asthma. NGF antibody may be a new therapeutic approach to asthma by inhibiting the expression of TNFα mRNA in the lower respiratory tract and visceral afferent organs during asthma.