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目的观测3种抗疟药对疟原虫诱导的大鼠腹腔巨噬细胞释放肿瘤坏死因子（TNF）的影响。方法：以酶联免疫法检测细胞培养液中TNF的含量。结果：感染约氏疟原虫的红细胞可诱导大鼠腹腔巨噬细胞释放TNF，12h时释出量达高锋。氯喹、硝喹明显抑制TNF的释放，乙胺嘧啶作用不明显。结论：氯喹、硝喹抑制TNF释放可能是其抗疟作用机制之一。
Objective To observe the effects of three kinds of antimalarial drugs on the release of tumor necrosis factor (TNF) by parasite - induced peritoneal macrophages in rats. Methods: The content of TNF in cell culture medium was detected by enzyme-linked immunosorbent assay. Results: Erythrocytes infected with Plasmodium yoelii induced peritoneal macrophages to release TNF in rats and reached a high peak at 12 hours. Chloroquine, quinine significantly inhibited the release of TNF, pyrimethamine no obvious effect. Conclusion: The inhibition of TNF release by chloroquine and quinoline may be one of the mechanisms of its anti-malarial effect.