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目的:建立同时测定何首乌中10种核苷类成分的QTRAP UPLC-MS/MS分析方法,分析比较生何首乌和制何首乌中核苷类成分的差异。方法:采用QTRAP UPLC-MS/MS技术,Waters Atlantis T3色谱柱(2.1 mm×150 mm,3μm),流动相甲醇-5 mmol·L-1乙酸铵(含0.1%冰乙酸),0.4 m L·min-1梯度洗脱,采用正离子多反应监测模式测定何首乌商品药材中10种核苷类成分的含量。结果:10种核苷类成分具有良好的线性关系,相关系数均>0.99;何首乌商品药材中核苷类成分尿苷、腺嘌呤、鸟苷、胞苷含量均较高;生何首乌与制何首乌中核苷类成分含量差异明显。结论:该方法简便、灵敏、准确。该研究为何首乌药材内在质量的评价和控制提供可靠的检测方法。
OBJECTIVE: To establish a method for the simultaneous determination of 10 nucleosides in Polygonum multiflorum by QTRAP UPLC-MS / MS, and to analyze the differences of nucleosides in Polygonum multiflorum and Polygonum multiflorum. Methods: A Waters Atlantis T3 column (2.1 mm × 150 mm, 3 μm) was used with mobile phase of methanol-5 mmol·L -1 ammonium acetate (containing 0.1% glacial acetic acid) and 0.4 mL · L -1 using QTRAP UPLC-MS / min-1 gradient elution, using positive ion multi-reaction monitoring mode determination of Polygonum multiflorum medicinal herbs 10 kinds of nucleoside content. Results: The 10 nucleoside components had a good linear relationship, the correlation coefficients were all> 0.99. The contents of uridine, adenine, guanosine and cytidine were higher in Polygonum multiflorum medicinal materials. Significant differences in the composition of the ingredients. Conclusion: The method is simple, sensitive and accurate. The study provides a reliable method for the evaluation and control of the intrinsic quality of Radix Polygoni multiflori.