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目的:观察钩藤与天麻配伍前后异构藤碱在SHR大鼠肝脏和肾脏的分布变化,探讨药物配伍前后对其归经的影响。方法:采用原发性高血压大鼠(SHR),钩藤组给予钩藤水煎液,天麻钩藤组给予钩藤天麻配伍后水煎液,空白组给予生理盐水,给药剂量为1.172 g.kg-1,用高效液相色谱法测定钩藤与天麻配伍前后其效应成分异钩藤碱在SHR大鼠肝脏和肾脏中的含量;色谱条件:色谱柱:WatersTM(4.6 mm×250 mm,RP 18.5μm);流动相甲醇-水(70∶30)pH 8.0;流速为0.8 mL.min-1,检测波长254 nm;柱温30℃;进样量20μL。结果:异钩藤碱对照品在肝脏和肾脏组织中在0.08～16.0 mg.L-1具有较好的线性关系;钩藤与天麻配伍前后在肝脏和肾脏的异钩藤碱的分布具有显著的差异性(P<0.05)。结论:中药配伍与归经之间有着密切的相关性。
OBJECTIVE: To observe the distribution of isofurantine in the liver and kidney of SHR rats before and after the combination of Uncaria rhynchophylla and Gastrodia elata, and to explore the influence of the drug before and after its compatibility. Methods: Primary hypertension rats (SHR) were used. The Uncaria rhynchophylla was given Uncaria decoction. The Uncaria rhynchophylla was given with the decoction of Uncaria rhynchophylla and the blank group with saline. The dosage was 1.172 g .kg-1, HPLC and HPLC were used to determine the contents of isorhamnetine in the liver and kidney of SHR rats before and after the combination of Uncaria and Tianma. Chromatographic conditions: WatersTM (4.6 mm × 250 mm, RP 18.5μm). The mobile phase was methanol-water (70:30) pH 8.0. The flow rate was 0.8 mL.min-1. The detection wavelength was 254 nm. The column temperature was 30 ℃ and the injection volume was 20μL. The results showed that the iso-rhynchophylline reference substance had a good linear relationship in the liver and kidney tissues at 0.08-16.0 mg.L-1. The distribution of isorhynchophylline in liver and kidney before and after compatibility of Uncaria rhynchophila and Gastrodia elata was significant Difference (P <0.05). Conclusion: There is a close correlation between traditional Chinese medicine compatibility and regression.