论文部分内容阅读
Objective:To analyze initially the differences of miRNAs expression profiles in human pancreatic cancer cell lines by microarray technique.Methods:A total of 743 probes were designed according to the known miRNAs sequences of human,mice,and rats.miRNAs microarray was manufactured and its credibility was verified.Total RNAs were extracted and miRNAs were separated from human pancreatic cancer cell lines(SW1990,Capan-2,BxPC-3,Aspc-1,and Panc1)and immortal human pancreatic duct epithelial cell line H6C7.They were labeled with T4 RNA ligase,then were hybridized with microarray.Through array scan and analysis,miRNAs expression profiles in pancreatic cancer were obtained.The results were verified by Northern blotting and RT-PCR.Results:A total of 63 miRNAs related to pancreatic cancer were found to be differentially expressed in 5 pancreatic cancer cell lines,including 25 down-regulated and 38 up-regulated miRNAs.Expres-sions of mir-21 and let-7 were also confirmed.Conclusion:The results suggested that miRNAs expression profiles could be found in pancreatic cancer cells.
Objective: To analyze initially the differences in miRNAs expression profiles in human pancreatic cancer cell lines by microarray technique. Methods: A total of 743 probes were designed according to the known miRNAs sequences of human, mice, and rats. MiRNAs microarray was manufactured and its .completeness of human pancreatic ductal epithelial cell line H6C7.They were labeled with human pancreatic cancer cell lines (SW1990, Capan-2, BxPC-3, Aspc-1, and Panc1) T4 RNA ligase, then were hybridized with microarray. Through array scan and analysis, miRNAs expression profiles in pancreatic cancer were. The results were verified by Northern blotting and RT-PCR. Results: A total of 63 miRNAs related to pancreatic cancer were found to be differentially expressed in 5 pancreatic cancer cell lines, including 25 down-regulated and 38 up-regulated miRNAs.Expres-sions of mir-21 and let-7 were also confirmed. Conlusion: The results sug gested that miRNAs expression profiles could be found in pancreatic cancer cells.