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应用ＰＣＲ甲基化分析法检测２５例原发性肝癌（ＨＣＣ）院标本及相应的癌旁组织中Ｐ１６基因甲基化情况。１资料与方法：２５例ＨＣＣ及相应的癌旁组织标本均为手术切除标本，置－８０℃冻存。参照《分子克隆》方法提取基因组Ｄ＼Ａ，测定Ａ２６０／Ａ２８０比值在１．８～２
PCR methylation analysis was used to detect methylation status of P16 gene in 25 cases of primary hepatocellular carcinoma (HCC) and corresponding paracancerous tissues. 1Materials and methods: 25 specimens of HCC and corresponding paracancerous tissue specimens were surgically resected and stored at -80°C. Refer to “Molecular cloning” method to extract genome DA and determine the ratio of A260/A280 to 1.8 to 2