在杂交瘤筛选过程中,为了纯化抗体阳性杂交瘤细胞,常需反覆进行克隆化。通常使用的克隆化方法是连续试管稀释法。该法准备器材较多,操作较繁,计算时易错。为了方便地进行单个细胞培养,利用9针头每40滴约1ml的特点试用“两管稀释法”,取得较好效果。现简要介绍于后:先取A管制 In the hybridoma screening process, in order to purify antibody-positive hybridoma cells, it is often necessary to perform cloning repeatedly. The commonly used cloning method is the continuous tube dilution method. The law to prepare more equipment, more complicated operation, calculation error-prone. In order to facilitate a single cell culture, the use of 9 needles for every 40 drops of about 1ml trial of “two-tube dilution method” to obtain better results. Now briefly introduced in the later: first take A regulation