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聚3-羟基丙酸[Poly(3-hydroxypropionate),P3HP]是一种生物可降解及生物相容的新型聚羟基脂肪酸酯。目前已鉴定的生物均不能天然合成P3HP。采用PCR克隆鼠伤寒沙门氏菌的丙醛脱氢酶(Pdu P)基因及罗尔斯通氏菌的聚羟基脂肪酸酯合成酶(Pha C)基因,构建共表达载体,转化肺炎克雷伯氏菌后获得两株重组菌。以甘油为唯一碳源进行摇瓶发酵,pdu P和pha C共用tac启动子的工程菌K.p(p ET-tac-pdu P-pha C)产生0.054 g/L的P3HP,而pdu P和pha C各自独用tac启动子的工程菌K.p(p ET-tac-pdu P-tac-pha C)产生0.091 g/L的P3HP。
Poly (3-hydroxypropionate, P3HP) is a novel biodegradable and biocompatible polyhydroxyalkanoate. Currently no organism has been identified for the natural synthesis of P3HP. PCR was used to clone the gene of Pdu P gene of Salmonella typhimurium and the polyhydroxyalkanoate synthase (Pha C) gene of the protothecoral of the protothecoides from the Staphylococcus aureus to construct a co-expression vector for transformation of Klebsiella pneumoniae After obtaining two strains of recombinant bacteria. The shake flask fermentation with glycerol as the sole carbon source resulted in 0.054 g / L P3HP for the engineering strain Kp (p ET-tac-pdu P-pha C) that shares the tac promoter with pdu P and pha C, whereas pdu P and pha C The engineered bacteria Kp (p ET-tac-pdu P-tac-pha C), each using the tac promoter alone, produced 0.091 g / L of P3HP.