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[目的]建立快速检测食源性致病菌的多重聚合酶链反应(PCR)方法。[方法]以出血性大肠杆菌的编码大肠杆菌不耐热毒素的B亚单位(LTB)基因、福氏志贺菌侵袭性质粒抗原H基因(ipaH)、霍乱弧菌肠毒素细胞外分泌蛋白(EPSM)基因作为目的基因,分别设计一对引物,优化各种工作条件,建立一种多重PCR诊断方法。[结果]应用所建立的多重PCR方法能快速、特异地检测出出血性大肠杆菌194bp、福氏志贺菌606bp、霍乱弧菌482bp的目的基因。[结论]该检测方法简单、快速、准确、灵敏度高,可广泛应用于食品卫生检测和临床检验等领域。
[Objective] To establish a rapid polymerase chain reaction (PCR) method for the detection of food-borne pathogens. [Method] The B subunit (LTB) gene encoding Escherichia coli heat-labile toxin, ipaH gene of Shigella flexneri, the extracellular secreted protein of Vibrio cholerae enterotoxin (EPSM) ) Gene as the target gene, a pair of primers were designed to optimize various working conditions, to establish a multiplex PCR diagnostic method. [Result] The multiplex PCR method was established to detect the target genes of 194bp, 606bp and 542bp of V. cholerae rapidly and specifically. [Conclusion] The method is simple, rapid, accurate and sensitive and can be widely used in the field of food hygiene testing and clinical testing.