【摘 要】
：
目的:观察电针干预对束缚应激模型大鼠行为学及血清中白细胞介素6(IL-6)与可溶性白细胞介素6受体(sIL-6R)表达的影响,探讨其相关免疫学机制.方法:将60只SD大鼠随机分为对照组
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目的:观察电针干预对束缚应激模型大鼠行为学及血清中白细胞介素6(IL-6)与可溶性白细胞介素6受体(sIL-6R)表达的影响,探讨其相关免疫学机制.方法:将60只SD大鼠随机分为对照组、模型组与电针组,每组20只.对照组群养;模型组采用孤养结合慢性束缚应激方式,每日束缚6h(9:00-15:00);电针组在每日束缚前给予电针刺激“百会”“印堂”;束缚期间3组均禁食禁水,其余时间自由饮食饮水.采用旷场和体质量实验进行行为学评价,并在第7天、28天、35天采用Bio-Plex检测大鼠血清中IL-6、ELISA检测SIL-6R水平.结果:①行为学:与对照组相比,模型组大鼠体质量及活动性均显著降低(P＜0.01),电针组大鼠体质量及活动性表达无显著差异(P＞0.05).②血清IL-6表达:实验day7,与对照组相比,模型组IL-6表达显著增加(P＜0.01),电针组IL-6表达无显著差异(P＞0.05);与模型组相比,电针组IL-6表达显著降低(P＜0.05).实验day28,与对照组相比,模型组IL-6表达显著增加(P＜0.01),电针组IL-6表达无显著差异(P＞0.05);与模型组相比,电针组IL-6表达显著降低(P＜0.01).实验day35,3组大鼠之间IL-6表达无显著差异(P＞0.05).③血清sIL-6R表达:实验day7,3组大鼠之间sIL-6R表达无显著差异(P＞0.05);实验day28,与对照组相比,模型组sIL-6R表达显著增加(P＜0.01),电针组sIL-6R表达无显著差异(P＞0.05);与模型组相比,电针组sIL-6R表达显著降低(P＜0.05).实验day35,3组大鼠之间sIL-6R表达无显著差异(P＞0.05).结论:慢性束缚应激模型大鼠血清IL-6、sIL-6R表达显著增加,并随时间出现相应变化,电针干预可对其进行显著调控,与行为学症状改善一致,这可能是电针抗抑郁的作用机制之一.
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