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目的 研究异丙托溴铵 (商品名 :溴化异丙托品 )对慢性缺氧大鼠气管平滑肌细胞大电导钙激活钾通道 (BKCa)的作用。方法 60只大鼠随机建立慢性大鼠缺氧模型组和正常组。急性分离单个大鼠气管平滑肌细胞。以膜片钳技术的内面向外式记录单通道电流。结果 (1 )采用内面向外式记录电流 ,慢性缺氧组通道开放概率 (P0 ,0 1 7± 0 0 7)与正常对照组 (0 35± 0 1 0 )比较 ,差异有显著性 (P <0 0 1 )。正常对照组快开放时间 (τO1 )为 (1 49± 0 41 )ms ,慢性缺氧组为 (0 53± 0 2 3)ms,正常对照组慢开放时间 (τO2 )为 (1 1 9± 3 2 )ms,慢性缺氧组为 (3 8± 1 4)ms,正常对照组快关闭时间(τc1 )为 (2 7± 0 9)ms,慢性缺氧组为 (5 7± 1 5)ms;正常对照组慢关闭时间 (τc2 )为 (1 2 1± 2 3)ms,慢性缺氧组为 (1 9 4± 2 9)ms ,两组快、慢、开、关闭时间比较差异有显著性 (P <0 0 1 )。异丙托溴铵与沙丁胺醇可逆转慢性缺氧对BKCa通道的抑制作用。通道的P0 慢性缺氧组为 0 1 5± 0 0 4、对照组为0 2 8± 0 0 9、沙丁胺醇组为 0 30± 0 0 8,三组间P0 比较差异有显著性 (P <0 0 1 )。慢性缺氧组τO1 、τO2 、τc1 和τc2 分别为 (0 55± 0 2 4 )ms、(3 6± 1 4)ms、(6 1± 1 6)ms、
Objective To investigate the effect of ipratropium bromide (ipratropium bromide) on the large-conductance calcium-activated potassium channel (BKCa) in trachea smooth muscle cells of rats with chronic hypoxia. Methods 60 rats were randomly divided into chronic hypoxia model group and normal group. Acute isolation of single rat tracheal smooth muscle cells. Single-channel current recording with Patch-Clamp technology. Results (1) With the inward-outward current recording, the open probability of channel opening in chronic hypoxia group (P0, 0 1 7 ± 0 0 7) was significantly higher than that in normal control group (0 35 ± 0 1 0) <0 0 1). The normal control group had a fast open time (τO1) of (1 49 ± 0 41) ms, a chronic hypoxic group of (533 ± 0 2 3) ms and a slow open time (τO2) of the normal control group (2 7 ms) in chronic hypoxia group, (38 ± 1 4) ms in chronic hypoxia group, (27 ± 0 9) ms in fasting hypoxia group, and ; The time of slow closure (τc2) was (121 ± 23) ms in normal control group and (194 ± 29) ms in chronic hypoxia group, there was significant difference between the two groups in fast, slow, open and close time Sex (P <0 0 1). Ipratropium bromide and albuterol can reverse the inhibitory effect of chronic hypoxia on BKCa channel. The difference of P0 between the three groups was significant (P <0, P0.01), and the difference was statistically significant (P0.01) 0 1). The values of τO1, τO2, τc1 and τc2 in chronic hypoxia group were (0 55 ± 0 2 4) ms, (36 ± 1 4) ms, (61 ± 1 6) ms,