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为了研究表皮生长因子受体（ｅｐｉｄｅｒｍａｌｇｒｏｗｔｈｆａｃｔｏｒｒｅｃｅｐｔｏｒ，ＥＧＦＲ）和Ｐ５３蛋白与膀胱肿瘤之间的关系，作者应用多重ＰＡＰ免疫细胞化学方法，通过增加ＰＡＰ的次数，提高了方法的敏感性，克服了抗体效价低和不适合在石蜡切片上染色的不足，对５８例石蜡包埋的膀胱肿瘤组织切片进行了检测．结果发现：ＥＧＦＲ的阳性率为３４．５％．它定位在细胞膜和胞浆内，而Ｐ５３蛋白的阳性率为２７．６％，主要定位在胞核内，少部分在胞浆内．它们的阳性率在肿瘤的病理分级和临床分期中差异显著（Ｐ＜０．０５），提示ＥＧＦＲ和Ｐ５３蛋白的异常表达与膀胱肿瘤的分化和浸润程度呈正相关．
In order to investigate the relationship between epidermal growth factor receptor (EGFR) and P53 protein and bladder tumor, the authors applied multiple PAP immunocytochemistry to increase the sensitivity of the method and to overcome the antibody titer by increasing the number of PAPs Low and unsuitable staining on paraffin sections of 58 cases of paraffin-embedded bladder tumor tissue sections were detected. The results showed that: the positive rate of EGFR was 34.5%. It is located in the cell membrane and cytoplasm, and P53 protein positive rate was 27.6%, mainly located in the nucleus, a small part of the cytoplasm. Their positive rates were significantly different between the pathological grade and clinical stage (P <0.05), which indicated that the abnormal expression of EGFR and P53 protein was positively correlated with the differentiation and infiltration of bladder tumor.