应用基因重组技术将抗人活化血小板α颗粒膜蛋白GMP-140单克隆抗体SZ-51可变区基因片段与人免疫球蛋白γ1重链CH1和κ轻链恒区基因进行拼接,应用PCR方法扩增上述重组基因片段,构建噬菌体质粒pHEN1 SZ-51Fab/Hu表达载体,将pHEN1-51Fab/Hu The gene fragment of GFP-140 monoclonal antibody against human activated platelet α-granule membrane protein (GMP-140) was fused with human immunoglobulin γ1 heavy chain CH1 and κ light chain constant region genes by gene recombination technique and PCR amplification The above recombination gene fragment was added to construct phage plasmid pHEN1 SZ-51Fab / Hu expression vector, pHEN1-51Fab / Hu