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目的:探讨miR-143在不同程度宫颈病变组织中的表达及其与临床病理特征的关系。方法:收集不同程度宫颈病变组织标本共140例,其中HPV(-)宫颈炎23例、HPV(+)宫颈炎21例、CINⅠ级28例、CINⅡ~Ⅲ级25例、宫颈鳞状细胞癌43例。用Real-time PCR方法检测不同程度宫颈病变组织中miR-143的表达,并分析其表达与不同程度宫颈病变临床病理特征的关系。结果:miR-143在宫颈鳞癌组织中的表达均显著低于宫颈炎组织、CINⅠ级和CINⅡ~Ⅲ级,差异有统计学意义(P<0.05);小细胞型宫颈癌的miR-143表达明显低于其他病理类型的标本,差异有统计学意义(P<0.01);不同分化程度的宫颈鳞癌miR-143表达差异有统计学意义(P<0.05)。结论 :miR-143在宫颈鳞癌组织中呈低表达,可能与宫颈鳞癌的发生和发展有关,有望成为宫颈鳞癌新的预后指标及治疗靶点。
Objective: To investigate the expression of miR-143 in different degree of cervical lesions and its relationship with clinicopathological features. Methods: A total of 140 cases of cervical lesions were collected, including 23 cases of HPV (-) cervicitis, 21 cases of HPV (+) cervicitis, 28 cases of CINⅠgrade, 25 cases of CINⅡ ~ Ⅲgrade, 43 cases of cervical squamous cell carcinoma example. Real-time PCR was used to detect the expression of miR-143 in different degree of cervical lesions, and to analyze the relationship between the expression of miR-143 and clinicopathological features of cervical lesions. Results: The expression of miR-143 in cervical squamous cell carcinoma was significantly lower than that in cervicitis, CINⅠ and CINⅡ ~ Ⅲ, the difference was statistically significant (P <0.05); miR-143 expression in small cell cervical cancer Significantly lower than other pathological types of specimens, the difference was statistically significant (P <0.01); different degrees of differentiation of cervical squamous cell carcinoma miR-143 expression difference was statistically significant (P <0.05). Conclusion: The low expression of miR-143 in cervical squamous cell carcinoma may be related to the occurrence and development of cervical squamous cell carcinoma, which is expected to become a new prognostic indicator and therapeutic target for cervical squamous cell carcinoma.