在进行松树线虫疫情调查和进境木质辅垫材料线虫检疫时 ,常需要将线虫制成临时玻片 ,在显微镜下观察鉴定。就伞滑刃线虫Ｂｕｒｓａｐｈｅｌｅｎｃｈｕｓ雄虫来说 ,其端生交合伞的观察 ,在鉴定上具有重要意义。正常情况下 ,雄虫杀死后侧面呈“Ｊ”形 ,观察到的尾部呈鸡爪状 ,很 In the investigation of pine nematode epidemic and quarantine of imported wooden auxiliary cushion material nematodes, nematodes often need to make a temporary slide, observed under a microscope identification. In the case of the Bursaphelenchus male, the observation of its terminal mating umbrella is of great importance in the identification. Under normal circumstances, the male killed after the back was “J” -shaped, the tail was observed chicken claw-like, very