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目的分析中介体19亚基(Med19)表达与乳腺癌临床病理特征的关系。方法用RT-PCR检测Med19基因在6株人乳腺癌细胞ZR-75-30、MDA-MB-231、SK-BR-3、T-47D、HCC-1937及MCF-7中的表达。用免疫组化方法检测Med19蛋白在150例临床乳腺癌组织及癌旁正常组织中的表达并分析其与临床病理特征的关系。结果 Med19基因在人乳腺癌细胞株ZR-75-30、MDA-MB-231、SK-BR-3、T-47D、HCC-1937及MCF-7中均有较高丰度的阳性表达。乳腺癌组织中Med19蛋白阳性表达率为52.0%,明显高于癌旁正常组织的9.3%(P<0.01)。Med19蛋白的阳性表达与乳腺癌病理高分级相关(P<0.01),未发现与患者年龄、肿瘤大小、病理组织类型、TNM分期、雌激素受体(ER)、孕激素受体(PR)、p53有统计学意义的相关性(P>0.05)。结论 Med19在乳腺癌组织中高表达,可能在乳腺癌的发生发展中起重要的作用。
Objective To analyze the relationship between the expression of Med19 and the clinicopathological features of breast cancer. Methods The expression of Med19 gene in six human breast cancer cell lines ZR-75-30, MDA-MB-231, SK-BR-3, T-47D, HCC-1937 and MCF-7 was detected by RT- Immunohistochemistry was used to detect the expression of Med19 protein in 150 cases of clinical breast cancer tissues and adjacent normal tissues, and to analyze its relationship with clinicopathological features. Results Med19 gene was highly expressed in human breast cancer cell lines ZR-75-30, MDA-MB-231, SK-BR-3, T-47D, HCC-1937 and MCF-7. The positive expression rate of Med19 protein in breast cancer tissues was 52.0%, which was significantly higher than that in the adjacent normal tissues (9.3%, P <0.01). The positive expression of Med19 protein was correlated with the high grade of breast cancer (P <0.01). No correlation was found between the expression of Med19 protein and the age, tumor size, histological type, TNM staging, estrogen receptor (ER), progesterone receptor p53 was statistically significant (P> 0.05). Conclusion The high expression of Med19 in breast cancer tissues may play an important role in the development of breast cancer.