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目的:观察N-甲基-D-天冬氨酸受体2亚基B(NR2B)和突触后致密物95(PSD-95)蛋白在锂-匹罗卡品致大鼠海马表达的动态变化,探讨其在颞叶癫癎发生、发展中的作用。方法:采用锂-匹罗卡品颞叶癫癎大鼠模型,用免疫组织化学法观察不同时间点大鼠海马CA1、CA3、DG区NR2B和PSD-95蛋白表达。结果:NR2B和PSD-95蛋白在大鼠海马分布广泛,表达丰富;大鼠腹腔注射锂-匹罗卡品后,NR2B和PSD-95蛋白在海马各区表达逐渐减少,24h降至低谷,与对照组比较差异有显著意义(P<0.01);此后逐渐回升,但仍低于对照组;30d在海马CA1、CA3区表达再次降低,与对照组比较有显著意义(P<0.05)。结论:NR2B和PSD-95蛋白表达下调可能分别参与颞叶癫癎急性期和慢性自发发作期的保护性机制。
AIM: To observe the dynamic changes of NR2B and PSD-95 proteins in hippocampus induced by lithium-pilocarpine-induced rat N-methyl-D-aspartate receptor 2 Changes, to explore its temporal lobe epilepsy, the role of development. Methods: The model of temporal lobe epilepsy was induced by lithium-pilocarpine. The expression of NR2B and PSD-95 protein in hippocampal CA1, CA3 and DG of hippocampus were observed by immunohistochemistry at different time points. Results: NR2B and PSD-95 proteins were widely distributed and abundant in the hippocampus of rats. After intraperitoneal injection of lithium-pilocarpine, the expression of NR2B and PSD-95 decreased gradually in hippocampus and decreased to the lowest level in 24 h (P <0.01), but then gradually recovered but still lower than that of the control group. The expression of CA1 and CA3 in the hippocampus decreased again on the 30th day, which was significantly lower than that of the control group (P <0.05). Conclusion: The down-regulation of NR2B and PSD-95 protein may participate in the protective mechanisms of temporal lobe epilepsy and chronic spontaneous seizure.