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[目的]观察大学生人群对常用增塑剂酞酸二异辛酯[d(i2-ethylhexyl)phthalate,DEHP]及酞酸二丁酯(di-n-butyl phthalate,DBP)的暴露情况,了解DEHP水平与男性血清雌二醇(estrogen,E2)、睾酮(testosterone,T)之间的关系。[方法]选择某大学沪籍新生420名为研究对象(18～19岁,男216人,女204人),静脉采集血清样本,使用气相色谱-质谱联用仪(GC-MS)测定血清中酞酸酯水平。样品预处理采用正己烷液相提取,洗脱采用温度程序,标准曲线采用五点法。采用全自动酶联免疫吸附及化学发光反应测定激素水平。[结果]DEHP、DBP的检出限均为0.1μg/L;二者回收率分别为91.3%～94.0%和91.2%～94.1%;变异系数分别为0.49%～2.79%和0.61%～3.42%;线性范围1～10000μg/L;背景污染未检出。420份血清中有88份(男41份,女47份)DEHP浓度高于检出限;检出样品的平均浓度(M)为54.14μg/L,四分位数间距(QR)为40.22μg/L。检出率及浓度在性别间差异无统计学意义(P>0.05)。DBP仅检出7份(男1份,女6份);检出样品平均浓度M为10.17μg/L,QR为9.30μg/L。高于检出限的41名男性(检出组)的DEHP浓度与T水平呈正相关关系(rs=0.347,P=0.048)。与低于检出限的59名男性(未检出组)相比,检出组的E2水平较高(P=0.035)。随着E2浓度升高,DEHP也趋向于被检出(β=-0.017,OR=0.983,P=0.021)。[结论]该人群DEHP检出率明显高于DBP,男性DEHP浓度与T水平呈正相关关系。E2浓度与男性DEHP检出与否有关,检出组E2浓度高于未检出组。
[Objective] To observe the exposure of common college plasticizer d (i2-ethylhexyl) phthalate (DEHP) and di-n-butyl phthalate (DBP) Serum levels of estrogen (E2) and testosterone (T) in male patients. [Methods] A total of 420 freshmen were selected as the study subjects (18-19 years old, 216 males and 204 females). Serum samples were collected intravenously and analyzed by gas chromatography-mass spectrometry (GC-MS) Phthalate level. Sample pretreatment n-hexane liquid phase extraction, elution temperature program, the standard curve using five-point method. The level of hormone was determined by automatic enzyme-linked immunosorbent assay and chemiluminescence reaction. [Results] The detection limits of DEHP and DBP were both 0.1μg / L. The recoveries of the two methods were 91.3% ~ 94.0% and 91.2% ~ 94.1%, respectively. The coefficients of variation were 0.49% ~ 2.79% and 0.61% ~ 3.42% ; Linear range of 1 ~ 10000μg / L; background pollution was not detected. 88 of the 420 sera (41 males and 47 females) showed a DEHP concentration higher than the limit of detection (LOD). The average concentration of the samples (54.14 μg / L) and the interquartile range (QR) was 40.22 μg / L. There was no significant difference in the detection rate and concentration between the sexes (P> 0.05). DBP only detected 7 copies (male 1, female 6); the average concentration of detected samples M was 10.17μg / L, QR was 9.30μg / L. There was a positive correlation between DEHP concentration and T level in 41 men above the detection limit (rs = 0.347, P = 0.048). The E2 level in the test group was higher (P = 0.035) compared with 59 men below the limit of detection (not detected). DEHP also tended to be detected with increasing E2 concentration (β = -0.017, OR = 0.983, P = 0.021). [Conclusion] The detection rate of DEHP in this population was significantly higher than that of DBP. There was a positive correlation between DEHP concentration and T level in males. E2 concentration and male DEHP detection is related to the detection group E2 concentration was higher than the undetectable group.