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粳型短日不育新种质5021S育性转换表现为“长光高温下可育,短光低温下不育”的反(向)光、温敏核不育特性。以5021S/轮回422组合F2作为遗传群体进行育性相关基因的QTL定位,利用124对SSR标记构建了1张全长1 912.3 cM、平均图距为14.98 cM的饱和分子连锁图谱。利用Windows QTL Cartographer 2.5软件检测分析,在全基因组范围内检测到4个控制育性相关性状的QTLs位点,分别位于第2,3,5,7染色体上,单个QTL可解释4.5%～32.5%表型变异,其中位于第2染色体上RM5804与RM425之间的qpms-2贡献率为32.5%,第3染色体RM130与RM3405之间的qpms-3贡献率为16.1%。表明5021S的核不育性受2对隐性主基因控制,同时受微效基因调控。
The fertility translocation of new japonica-type short-fertile male sterile line 5021S was characterized by reverse (backward) light and thermo-sensitive genic male sterility “fertile at long light and high temperature, sterility under short light and low temperature”. A total of 1 912.3 cM full-length molecular marker linkage map with 14.98 cM full-length map was constructed using 124 pairs of SSR markers, using 5021S / reincarnation 422 combinations F2 as genetic groups. Four QTLs loci controlling fertility-related traits were detected in the genome-wide range on chromosomes 2, 3, 5, and 7 using a QTL Cartographer 2.5 software. A single QTL explained 4.5% -32.5% Among them, qpms-2 contributed 32.5% between RM5804 and RM425 on chromosome 2, and qpms-3 contribution between RM130 and RM3405 on chromosome 3 was 16.1%. The results showed that the nuclear sterility of 5021S was controlled by two pairs of recessive major genes and regulated by minor genes.