论文部分内容阅读
【目的】建立高效的杉木炭疽菌遗传转化体系,并观察附着胞发育过程中的细胞核行为。【方法】通过菌丝体酶解的方法制备原生质体,PEG介导的原生质体转化法将含有伯莱霉素抗性的细胞核表达质粒NL1∶∶GFP转入受体材料杉木炭疽菌SMCG1#C菌株,通过荧光显微镜跟踪观察附着胞发育过程中的孢子、芽管和附着胞等结构中的细胞核行为。【结果】获得了稳定表达NL1∶∶GFP质粒的杉木炭疽菌阳性转化子;杉木炭疽菌附着胞发育过程伴随着细胞的有丝分裂与细胞核的转移;附着孢发育成熟后,孢子和芽管菌丝中的细胞核仍然保持完整。【结论】建立了高效的杉木炭疽菌遗传转化体系;杉木炭疽菌与稻瘟病菌、希金斯炭疽菌的附着胞发育过程的细胞生物学特性显著不同。
【Objective】 The objective of this study was to establish an efficient genetic transformation system for C. solani and to observe the nuclear behavior of adherent cells. 【Method】 Protoplasts were prepared by mycelial enzymatic digestion. The protoplast transformation mediated by PEG was used to transfer the propamycin-resistant cell nucleus expression plasmid NL1:GFP into the recipient material Fusarium venenatum SMCG1 # C Strain, observed by fluorescence microscopy attached apples during the development of the spores, germ tube and appressorium nucleus structure and other behavior. 【Result】 The positive clones of C. gossypii were obtained by stably expressing NL1:GFP plasmid. The attachment of Cunninghamia lanceolata was accompanied by cell mitosis and nuclear transfer. After the conidial spore matured, The nucleus remains intact. 【Conclusion】 The establishment of an efficient transformation system of Chinese fir anthracnose had significant differences in cell attachment and development between C. grisea and M. grisea and Higgins anthracnose.