论文部分内容阅读
背景分形素Fractalkine(FKN)是新近发现的唯一兼具黏附功能的趋化因子,不仅能够诱导单核细胞的定向迁移,还能介导单核细胞与血管内皮细胞的紧密黏附,参与动脉粥样硬化(AS)的形成。目的观察白细胞介素18(IL-18)诱导人脐静脉内皮细胞(HUVEC)上调表达FKN后,FKN介导的HUVEC与人单核细胞细胞株1(THP-1)的黏附。方法应用逆转录聚合酶链反应(RT-PCR)检测HUVEC FKN mRNA的表达,应用FlowChamber装置观察HUVEC与人单核细胞THP-1的黏附,以及FKN中和抗体对黏附效应的影响。结果IL-18可剂量相关地诱导FKN mRNA表达,与0μg/L组(0.10±0.01)相比,25、50、100μg/L组分别增加为:(0.49±0.04),(0.63±0.09),(0.85±0.07)(均P<0.01)。FKN表达上调相应地明显增加HUVEC与单核细胞的黏附,单核细胞黏附数在0、25、50、100μg/L IL-18组,分别为:(7.4±2.6),(26.8±5.2)、(43.0±5.4)、(70.8±10.7)/HP(均P<0.01)。FKN中和抗体可明显抑制此种黏附效应。结论FKN在介导HUVEC与单核细胞的黏附过程中发挥作用。
Background Fractal fractalkine (FKN) is the newly discovered chemokine with adhesion function. Not only can it induce the directional migration of monocytes, but also induce the close adhesion between monocytes and vascular endothelial cells, Hardening (AS) formation. Objective To observe the adhesion of HUVEC to human monocytic cell line 1 (THP-1) induced by interleukin 18 (IL-18) induced by up-regulated expression of FKN in human umbilical vein endothelial cells (HUVECs). Methods The expression of FKN mRNA in HUVECs was detected by reverse transcriptase polymerase chain reaction (RT-PCR). The adhesion of HUVEC to human monocytic THP-1 and the effect of FKN neutralizing antibody on adhesion were observed by flow cytometer. Results IL-18 induced a dose-dependent increase in FKN mRNA expression in the 25, 50 and 100 μg / L groups compared to the 0 μg / L group (0.49 ± 0.04 vs. 0.63 ± 0.09, (0.85 ± 0.07) (all P <0.01). The up-regulation of FKN significantly increased the adhesion of HUVEC to monocytes. The number of monocyte adhesion was (7.4 ± 2.6), (26.8 ± 5.2), (43.0 ± 5.4) and (70.8 ± 10.7) / HP (all P <0.01). FKN neutralizing antibody significantly inhibited this adhesion effect. Conclusion FKN plays a role in the adhesion between HUVEC and monocytes.