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目的:观察机械牵张应变作用下,人牙周膜细胞(HPDLCs)内磷脂酶C-γ1(PLC-γ1)水平的变化。方法:通过原代和传代培养,获得性状稳定的人牙周膜细胞,使用动态机械应变细胞加载仪对细胞进行1%、10%和20%动态牵张应变加载。加载不同时间,通过流式细胞仪对细胞进行PLC-γ1水平检测。采用SPSS13.0软件包对数据进行方差分析。结果:在加载牵张应变初期,细胞内的PLC-γ1水平维持较低水平;随着牵张应变加载时间的延长,牙周膜细胞内的PLC-γ1水平逐渐升高。50min时,PLC-γ1水平达到各应变组的最高值(P<0.01)。随着加载时间的继续延长,60min时,各应变组的PLC-γ1水平都显著下降(P<0.01)。结论:牵张应变可引起细胞内PLC-γ1水平的变化。
Objective: To observe the changes of phospholipase C-γ1 (PLC-γ1) in human periodontal ligament cells (HPDLCs) under mechanical stretch stress. Methods: Human periodontal ligament cells with stable morphology were obtained by primary and subculture. The cells were loaded with dynamic stretch strain of 1%, 10% and 20% using dynamic mechanical strain cell loading instrument. Loaded at different times, PLC-γ1 levels were detected by flow cytometry. Data were analyzed for variance using SPSS 13.0 software package. Results: The PLC-γ1 level in the cells maintained a relatively low level at the initial stage of strain loading. The PLC-γ1 level in periodontal ligament cells gradually increased with the extension of stretch strain. At 50 min, the level of PLC-γ1 reached the highest value in each strain group (P <0.01). With the prolongation of loading time, PLC-γ1 levels in all strain groups decreased significantly at 60min (P <0.01). Conclusion: Stretching strain can cause changes in intracellular PLC-γ1 levels.