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将携带有人粒细胞-巨噬细胞集落刺激因子（GM—CSF）基因的重组腺病毒载体转集BAI．B／c小鼠肝癌细胞株（H22），体外经60Coγ射线灭活后制成瘤苗，用以治疗荷瘤小鼠。应用流式细胞仪（FCM）测定荷瘤小鼠外周血T淋巴细胞亚群的变化，并观察荷瘤小鼠生存期。结果发现，GM－CSF基因转染瘤苗能显著提高小鼠外周血CDs8＋T淋巴细胞亚群的含量，荷瘤小鼠生存期显著延长。结果表明：GM—CSF基因转染瘤苗能诱导出有效的抗肿瘤免疫反应，提示应用该疗法进行肿瘤基因治疗的可行性。
The recombinant adenoviral vector carrying human granulocyte-macrophage colony-stimulating factor (GM-CSF) gene was transferred to BAI. B/c mouse hepatocellular carcinoma cell line (H22) was inactivated by 60Co γ-ray in vitro and then used to treat tumor-bearing mice. Flow cytometry (FCM) was used to measure the changes of peripheral T lymphocyte subsets in tumor-bearing mice and the survival of tumor-bearing mice was observed. The results showed that the GM-CSF gene transfected tumor vaccine can significantly increase the content of CDs8+ T lymphocyte subsets in mice peripheral blood, and the survival period of tumor-bearing mice was significantly prolonged. The results showed that: GM-CSF gene transfected tumor vaccine can induce effective anti-tumor immune response, suggesting the feasibility of this therapy for tumor gene therapy.