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目的为了获得浙江省汉坦病毒基因组更为详尽的资料,研究汉坦病毒的进化状况及变异程度,为疫苗病毒株的选择使用提供科学依据。方法本实验室利用RT-PCR方法扩增ZT71株S基因片段并克隆入质粒载体,进行核苷酸序列测定及分析。结果ZT71株S基因由1754个核苷酸组成,只有一个开放读码框架,共编码429个氨基酸。与HTN型病毒(76-118)的核苷酸和氨基酸同源性分别为72.0%和82.6%,与SEO型病毒(SR-11、R22、Guo3、8610)核苷酸和氨基酸同源性分别为88.4%—96.5%和98.1%—99.0%,与其它型汉坦病毒的同源都很低。结论表明此分离的病毒为SEO型汉坦病毒,为进一步研究其进化和变异提供了有利条件。
Objective To obtain more detailed information about the Hantaan virus genome in Zhejiang Province, to study the evolution and variation of Hantavirus and to provide a scientific basis for the selective use of the vaccine strain. Methods The S gene fragment of ZT71 strain was amplified by RT-PCR in our laboratory and cloned into plasmid vector for nucleotide sequence determination and analysis. Results The S gene of ZT71 strain consisted of 1754 nucleotides, only one open reading frame, encoding a total of 429 amino acids. Nucleotide and amino acid homologies with HTN-type viruses (76-118) were 72.0% and 82.6%, respectively, and those with SEO-type viruses (SR-11, R22, Guo3 and 8610) 88.4% -96.5% and 98.1% -99.0%, all with low homology to other Hantavirus strains. The results showed that the isolated virus was SEO-type Hantavirus, which provided favorable conditions for the further study of its evolution and mutation.