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目的:研究连豆清脉颗粒对日本大耳白兔动脉粥样硬化(AS)斑块的干预作用及多聚二磷酸腺苷核糖聚合酶-1(poly adenosine diphosphate ribose polymerase-1,PARP-1)的影响。方法:取健康清洁级雄性日本大耳白兔40只,随机抽取8只日本大耳白兔为空白组,以标准饲料喂养。其余32只日本大耳白兔采用高脂饲料喂养及牛血清白蛋白静脉注射建立AS斑块模型,成功建模的兔按照随机数字表,随机分成高脂组11只(实验过程中死亡2只)、连豆清脉方组8只,辛伐他汀组8只,分别予以高脂、连豆清脉方药物、辛伐他汀药物饲料喂养,给药剂量为辛伐他汀1 mg·kg~(-1)·d~(-1),连豆清脉方3.7 g·kg~(-1)·d~(-1),8周后,检测兔AS斑块面积,PARP-1阳性表达及Toll样受体-4(Toll like receptor-4,TLR-4)mRNA,核转录因子-κB(nuclear factor kappa-B,NF-κB),血清白细胞介素-6(interleukin-6,IL-6)水平。结果:空白组没有出现AS斑块,连豆清脉方组AS斑块明显,但是明显低于高脂组;与空白组比较,连豆清脉方组主动脉AS斑块面积比例,组织PARP-1阳性细胞表达率,TLR-4 mRNA表达,NF-κB及IL-6水平均显著升高(P<0.01)。与高脂组比较,连豆清脉方组主动脉AS斑块面积比例,组织PARP-1阳性细胞表达率,TLR-4 mRNA表达,NF-κB及IL-6水平显著下降(P<0.01)。结论:连豆清脉方可抑制AS斑块的增生;其机制可能与抑制PARP-1表达等炎症反应有关。
Objective: To study the intervention of Jiedou Qingmai Granule on atherosclerosis (AS) plaques of Japanese white rabbits and the effect of poly adenosine diphosphate ribose polymerase-1 (PARP-1) )Impact. Methods: Forty male Japanese white rabbits with healthy and clean grade were randomly selected. Eight Japanese white rabbits were randomly selected as blank group and fed with standard diet. The remaining 32 Japanese white rabbits were fed with high-fat diet and bovine serum albumin intravenously to establish the AS plaque model. The rabbits successfully modeled were randomly divided into 11 groups according to a random number table: 2 in the hyperlipidemia group (2 in the experiment ), 8 in the Liantang Recipe group, and 8 in the Simvastatin group were fed with high fat, Loudou Recipe and Simvastatin respectively. The rats were given simvastatin 1 mg · kg ~ (-1) (-1) · d ~ (-1) and 3.7 g · kg ~ (-1) · d ~ (-1) respectively. After 8 weeks, the area of plaque, the expression of PARP-1 and the expression of PARP- Toll-like receptor 4 (TLR-4) mRNA, nuclear factor kappa-B (NF-κB), interleukin-6 )Level. Results: There were no AS plaques in the blank group, and the AS plaque in the Liantou Qingmai prescription group was significantly lower than that in the high-fat diet group. Compared with the blank control group, the AS plaque area proportion in the aorta and the PARP 1 positive cells, TLR-4 mRNA expression, NF-κB and IL-6 levels were significantly increased (P <0.01). Compared with the hyperlipidemia group, the proportion of AS plaque area, the expression of PARP-1 positive cells, the expression of TLR-4 mRNA, the levels of NF-κB and IL-6 in Liaozhuanmai prescription group decreased significantly (P <0.01) . Conclusion: Liantao Qingmai can inhibit the proliferation of AS plaque; its mechanism may be related to the inhibition of PARP-1 expression and other inflammatory responses.