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为探索人乳头状瘤病毒（ＨＰＶ）感染与四川盐亭地区食管癌高发的病因学关系，用生物素标记的ＨＰＶ１６ＤＮＡ探针对盐亭食管癌高发区食管癌、正常食管上皮及成都食管癌低发区正常食管上皮脱落细胞进行原位杂交，检测ＨＰＶ１６ＤＮＡ。结果：盐亭地区食管癌及正常食管上皮ＨＰＶ１６ＤＮＡ阳性率分别为５０．５％（２０／４０）和６２．１％（３６／５８），两者无显著性差异（Ｐ＞０．０５）；成都地区正常食管上皮ＨＰＶ１６ＤＮＡ阳性率为１５．４％（６／３９），低于盐亭地区正常食管上皮的阳性率，有显著性差异（Ｐ＜０．０１）。研究结果表明，ＨＰＶ感染与四川盐亭地区食管癌高发有关。
To explore the etiology of human papillomavirus (HPV) infection and the high incidence of esophageal cancer in Yanting, Sichuan, biotin-labeled HPV16 DNA probes were used to lower esophageal cancer, normal esophageal epithelium, and Chengdu esophageal cancer in high-risk esophageal cancer areas in Yanting. The normal esophageal epithelial exfoliated cells in the hair area were used for in situ hybridization to detect HPV16 DNA. Results: The positive rates of HPV16 DNA in esophageal cancer and normal esophageal epithelium in Yanting were 50.5% (20/40) and 62.1% (36/58) respectively, there was no significant difference between the two (P>0.05). The positive rate of HPV16 DNA in normal esophageal epithelium in Chengdu was 15.4% (6/39), which was lower than the positive rate of normal esophageal epithelial in Yanting area, with significant difference (P<0.01). The results of the study indicate that HPV infection is associated with high incidence of esophageal cancer in Yanting, Sichuan.