七十年代出现的HRP轴浆运输标记法对神经解剖学作出了重要贡献。但神经元对“单纯HRP”(FHRP)只是作非特异性的吞饮,胞浆溶酶体随即将其分解,因而大大限制了其逆行,顺行传送标记的灵敏性。应用细胞膜上各种受体对某些植物凝聚素或细菌毒素有特异性结合的原理,我们以六种植物凝聚素和二种细菌毒素分别同HRP相结合,来提高对神经元标记的灵敏度。用相当于5微克HRP溶于1微升磷酸缓冲液内的各种“结合HRP”或 In the seventies, HRP axonal transport markers have made important contributions to neuroanatomy. However, neurons only nonspecific swallowing of “pure HRP” (FHRP), which is then immediately disassembled by cytoplasmic lysosomes, which greatly limits the sensitivity of retrograde and forward delivery of labels. Using the principle that various receptors on the cell membrane bind specifically to certain plant lectins or bacterial toxins, we combined six plant lectins and two bacterial toxins, respectively, with HRP to increase sensitivity to neuronal markers. Various “binding HRPs” or 5 mg HRP dissolved in 1 microliter of phosphate buffer or