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目的:研究肝外胆管癌组织中细胞凋亡相关基因Fas和FasL的表达及意义。方法:采用免疫荧光组织化学方法结合激光扫描共聚焦显微镜对30例肝外胆管癌及10例胆总管囊肿组织进行观察。结果:30例肝外胆管癌中,Fas和FasL阳性21例,阳性率70%,对照组10例胆总管囊肿Fas和FasL阳性1例,阳性率10%,Fas和FasL在肝外胆管癌中的表达强于对照组(P<0.05);同一肿瘤细胞中Fas定量大于FasL(P<0.05);Fas和FasL主要位于胞浆和胞核。结论:Fas和FasL参与了大部分肝外胆管癌的凋亡调控,但肝外胆管癌在发生分化过程中可能部分获得了逃逸Fas介导凋亡的功能。
Objective: To study the expression and significance of apoptosis-related genes Fas and FasL in extrahepatic cholangiocarcinoma. Methods: Immunohistochemistry and laser scanning confocal microscopy were used to observe 30 cases of extrahepatic cholangiocarcinoma and 10 cases of common bile duct cyst. RESULTS: In 30 cases of extrahepatic cholangiocarcinoma, Fas and FasL were positive in 21 cases, the positive rate was 70%. In the control group, 10 cases of Fas and FasL were positive in the common bile duct cyst. The positive rate was 10%. Fas and FasL were found in extrahepatic bile duct carcinoma. The expression of Fas and FasL was stronger in the cytoplasm and nucleus than that in the control group (P<0.05). Conclusion: Fas and FasL participate in the regulation of apoptosis in most extrahepatic cholangiocarcinomas. However, extracellular hepatic cholangiocarcinoma may partially escape the Fas-mediated apoptosis during differentiation.