尼罗红染色法可用于十二烷基硫酸钠(SDS)-聚丙烯酰胺凝胶中蛋白带的染色。与考马斯蓝和银染色不同,此法不需对凝胶中的蛋白进行固定。因此,蛋白带还可用于电印迹、测序或免疫学分析。尼罗红曾被用于脂类等物质疏水结构的染色,故其染色机理可能是作用于SDS-蛋白复合物中的疏水基团。 用Mini-Protean Ⅱ电泳仪进行蛋白电泳,将浓 Nile red staining can be used to stain protein bands in sodium dodecyl sulfate (SDS) -polyacrylamide gel. Unlike Coomassie blue and silver stains, this method does not require immobilization of the protein in the gel. Therefore, protein bands can also be used for electroblotting, sequencing or immunological analysis. Nile red has been used for lipid and other substances hydrophobic structure staining, so the dyeing mechanism may be acting on the SDS-protein complex hydrophobic groups. Using Mini-Protean Ⅱ electrophoresis protein electrophoresis, will be concentrated