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目的:研究卡马西平(Car)对γ-氨基丁酸(GABA)在海马区域作用的影响.方法:在海马脑片(350μm)上刺激(0.5 Hz,50μs)Schaffer氏纤维,记录CA1区锥体细胞的诱发场电位.结果:Car 0.2mmol·L~(-1)对CA1区锥体细胞的诱发场电位没有明显影响,但Car 0.2 mmol·L~(-1)和GABA(0.1-1 mmol·L~(-1))同用抑制场电位作用比单用GABA时显著增强.Bicuculline不能翻转被Car加强的GABA的抑制作用.继而,左旋巴氯芬抑制场电位作用强于GABA.Car 0.2 mmol·L~(-1)和左旋巴氯芬(1-5μmol·L~(-1))同用抑制场电位作用比单用左旋巴氯芬时显著增强.结论:Car增强GABA对海马CA1区锥体细胞的抑制作用,其作用机制可能与GABA_B受体有关.
Objective: To study the effect of Carbamazepine on the action of GABA in the hippocampus.Methods: Schaffer’s fibers (0.5 Hz, 50μs) were stimulated in the hippocampal slices (350μm) The results showed that Car 0.2 mmol·L -1 had no obvious effect on the induced field potential of CA1 pyramidal cells, but Car 0.2 mmol·L -1 and GABA 0.1-1 mmol·L -1) was significantly higher than that of GABA alone, while Bicuculline could not reverse the inhibitory effect of GABA enhanced by Car.Next, L-baclofen inhibited the field potential more strongly than GABA.Car 0.2 mmol·L -1 and L-baclofen (1-5 μmol·L -1) inhibited the field potentials significantly compared with L-baclofen alone.Conclusion: Carb increased the inhibitory effect of GABA on hippocampus CA1 pyramidal cells in the inhibition of its mechanism may be related to GABA_B receptors.