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本文主要研究了四个小麦品种不同长度的幼穗及幼穗不同穗段离体培养的效应.结果:(1)取长为0.1厘米、0.3厘米的幼穗全穗、或穗长为0.5厘米的幼穗上段或下段、或穗长为1.0厘米的幼穗上、中、下三段离体培养,愈伤组织诱导率均达100％;取穗长为2厘米的幼穗上、中、下段离体培养,愈伤组织诱导率分别为34％、80％和94％;取穗长为3厘米的幼穗上、中、下段离体培养,愈伤组织诱导率分别为14％、38％和58％.(2)用长1厘米以上和长0.1厘米的幼穗高体培养,其愈伤组织主要是分化根;将长0.3～0.5厘米的幼穗愈伤组织直接转移到分化培养基上,植株分化率为78.8％
In this study, the effects of in vitro culture on young spikelets and young spikelets of four wheat cultivars with different lengths were studied.Results: (1) Spikelets with spike length of0.1cm and0.3cm, or spike length of0.5cm Of the upper panicle or the lower panicle, or spike length of 1.0 cm in the upper, middle and lower three sections of in vitro culture, the callus induction rate reached 100%; take ear length of 2 cm young panicle, The in vitro callus induction rate was 34%, 80% and 94%, respectively. The callus induction rates of upper, middle and lower segments of spikelets were 3%, 14% and 38% respectively % And 58%, respectively. (2) The young callus with more than 1 cm in length and 0.1 cm in length had the callus mainly differentiated into roots. The young callus 0.3 ~ 0.5 cm in length was directly transferred to the differentiation culture On the basis of plant differentiation rate of 78.8%